Samples taken at different stages during the preparation of protein
particles were analysed using an optical microscope (Olympus
BX60) equipped with a DP71 digital camera (Olympus Corp. Centre
Valley, PA, USA). To record the images, each sample was gently
diluted with its continuous phase in a ratio of 1:1. A drop of diluted
sample was transferred onto the microscope slide and then covered
with a cover slip prior to analysis.