For TGGG hybrid grouper case in this study, it falls under fertility stage that shows gonads are normal in size and structure; gametes are also normal in size structure and finally zygotes are viable [24]. But still, for the current study, only sexual maturation was found and the viability of both oocyte and sperm of TGGG hybrid grouper has not yet been determined. Thus, other studies such as F2 generation [25,26] and backcross experiment [27,28] should be conducted to determine if the oocyte and sperm are viable.
For TGGG hybrid grouper case in this study, it falls under fertility stage that shows gonads are normal in size and structure; gametes are also normal in size structure and finally zygotes are viable [24]. But still, for the current study, only sexual maturation was found and the viability of both oocyte and sperm of TGGG hybrid grouper has not yet been determined. Thus, other studies such as F2 generation [25,26] and backcross experiment [27,28] should be conducted to determine if the oocyte and sperm are viable.
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