The data clearly showed that the fatty acid contents were highly
affected by the cold acclimatisation period. The total content of
fatty acids in kale subjected to cold acclimatisation were lower
(9–25%) after cold acclimatisation. Indeed the oleic acid (18:1)
fatty acid content was reduced by up to 91% (12 h; 15/9 C) after
cold acclimatisation whilst the content of behenic acid (22:0)
was uniformly higher (40%) (Table 3). The ratio between unsaturated
fatty acids vs. palmitic acid (16:0) + stearic acid (18:0) had a
higher portion of unsaturated fatty acids after cold acclimatisation
(Table 3) for all combinations of growth temperature and day
length, except the high growth temperature/24 h photoperiod.
The ratio between unsaturated fatty acids and all saturated fatty
acids did not appear to be impacted after cold acclimatisation, possibly
due to a higher content of behenic acid (22:0) after cold
acclimatisation.
3.3. Soluble sugars