Measurement of antioxidative activity
DPPH radical-scavenging activity
DPPH radical-scavenging activity was determined according to the method
of (Wu etal, 2003) with slight modifications. Briefly, 2ml of hydrolysates
sample was mixed with 2ml of 0.1 mM DPPH in 95% ethanol. The mixture
was shaken thoroughly and incubated for 30 min at room temperature in the
dark. The absorbance was determined wavelength of 517 nm. The scavenging
effect was quantified according to the following equation:
DPPH radical-scavenging activity (%)=[1-(A1-A2)/A0]×100
Where A1represents the absorbance of the sample with DPPH solution, A0
represents the absorbance of the control of the DPPH- ethanol solution and
A2 represents the absorbance of the sample added to 95% ethanol without
DPPH solution.