1 ml was injected in split injector (split ratio 100:1) set at 220 C.The
fatty acid methyl esters were separated in HP-88 capillary column
(Agilent, Las Rozas, Spain, 100 m, 0.25 mm i.d, 0.25 mm film thickness)
using helium at a flow rate of 26.03 cm/s.