GP-LRS3 exhibited the highest the
degree of ordered structure and the amorphous region of MPLRS3
was biggest by FT-IR and 13C NMR as well as UP-LRS3 displayed
the highest degree of double helical structure. These results
indicated that the structure of LRS3 was influenced by the preparation
methods. In addition, MP-LRS3 displayed the greatest solubility
and swelling power, which were affected by the amorphous
region, particle structure. UP-LRS3 exhibited the strongest iodine
absorption ability and thermostability, which were affected by
the degree of polymerization and degree of double helical structure,
respectively. The different physicochemical properties of
GP-LRS3, MP-LRS3 and UP-LRS3 were attributed to their different
structure. Interestingly, the property was a result of a combination
of multiple factors. The result will provide the background and
practical knowledge for the application of lotus seed resistant
starch. MP-LRS3 could be explored as a prebiotic and UP-LRS3
was a potential thermal stabilizer in food industries.