cyclesFig. 3. Re-use of beta-amylase glutaraldehyde-agarose derivative for several reac-tion cycles. The initial and remaining activities were assayed at pH 6.0, 50◦C (initialactivity is regarded as 100%). At the end of each cycle, the derivative was washedwith distilled water and activity buffer and a new substrate solution was added tostart a new round of reaction