Materials and Methods
Chemicals
Standard fatty acid methyl ester mixtures were
purchased from Sigma Chemical Co. (St. Louis, MO)
and stored at -20°C. All chemicals and solvents were
reagent grade and purchased from Sigma Chemical
Co. and Fisher Scientific, Inc. (Pittsburgh, PA).
Sample preparation
Twenty species of economically important marine
fish and 6 types of squid and shrimp (Table 1) were
collected from the fishing port of Pattani, Thailand.
Seven species of freshwater fish were purchased rom local markets. The samples of each species were
selected randomly, and collected every month for 10
months, from March through December. Samples
were put in plastic bags, kept in an icebox until turned
in to the laboratory within 1 h, and then kept at -20°C
in a freezer for not more than 24 h prior to further
analysis. Only edible muscle was used to determine
fat content and fatty acid composition. Each lot of
samples collected was analyzed in 3 replicates.
Materials and Methods
Chemicals
Standard fatty acid methyl ester mixtures were
purchased from Sigma Chemical Co. (St. Louis, MO)
and stored at -20°C. All chemicals and solvents were
reagent grade and purchased from Sigma Chemical
Co. and Fisher Scientific, Inc. (Pittsburgh, PA).
Sample preparation
Twenty species of economically important marine
fish and 6 types of squid and shrimp (Table 1) were
collected from the fishing port of Pattani, Thailand.
Seven species of freshwater fish were purchased rom local markets. The samples of each species were
selected randomly, and collected every month for 10
months, from March through December. Samples
were put in plastic bags, kept in an icebox until turned
in to the laboratory within 1 h, and then kept at -20°C
in a freezer for not more than 24 h prior to further
analysis. Only edible muscle was used to determine
fat content and fatty acid composition. Each lot of
samples collected was analyzed in 3 replicates.
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