Since SNPs are commonly believed to be associated with response to drug and disease outcome, one of the most valuable applications of SNPs would be a biomedical application such as disease diagnosis and therapeutics. Along this line, Purushothaman et al. [33] suggested an application of ISFET technology for the detection of SNPs. In that study, the authors developed a useful procedure for sequencing one base via the detection of single-base mismatch in DNA.