2.4. Data analysis
We performed individual paired t test to assess whether significant
differences could be detected between the total and volatile C
concentrations in the bark and stemwood tissue for each tree species.
We also used a partially nested analysis of variance (ANOVA)
to examine the effect of shade tolerance, species, and tree size
(DBH) on total and volatile C concentrations. Each tissue type
was analyzed separately using the following statistical model,
including two interaction terms, and tree species was nested
within shade tolerance, such as: