Biodiesel production from rubber seed by in situ method takes 120 minutes at 60o
C with maximum
yield of FAME 53.61% at H2SO4 0.25% (v/v) and maximum yield of FAME 91.05% at ratio of raw
material to methanol (1:3). Based on the results, ratio of raw material to methanol is quite important to
increase yield of FAME significantly.