The samples were then centrifuged (3000g for 10 min), and the
supernatant was recovered.
After two washings with minimum volumes of distilled water and recentrifuging as necessary, the final volume was taken up to 50 mL.
The tube was centrifugated at 3500g for 20 min and was transferred into tubes and directly used
for the determination of antioxidant capacity and total polyphenol
content.