CAMP test detects the production of diffusible, thermostable, extracellular protein known as CAMP factor, produced by Group B Streptococcus. The CAMP factor acts synergistically with the beta lysin produced by Staphylococcus aureus to produce a zone of enhanced lysis of sheep or bovine erythrocytes. The standard CAMP test depend on the elaboration of two toxins during growth to form a typical arrowhead or flame-shaped clearing at the junction of the two organisms when they are placed perpendicular to each other.