The developments in nanotechnology and microbiology have introduced a novel technique to immobilize active microbes of selected dominant microbial culture in nanofiber for effective remediation of pesticides. Vancov has demonstrated the atrazine degradation using alginate beads containing Rhodococcus erythropolis NI86/21 cells. His results kept breakthrough for suitable encapsulation of microorganism for herbicide removal (Vancov et al. 2007). Followed the above research, Sumana Siripattanakul and his coworkers have studied the atrazine degradation by immobilized pure and mixed cultures on PVA. The immobilized J14a and MC were shown approximately 50 and 40% of atrazine biodegradation within 120 h, respectively, at 3.5 mg/ml cell to matrix ratio (Siripattanakul et al. 2008). Lately, Shiri Klein studied the atrazine bioremediation using Pseudomonas sp. ADP cells encapsulated in electrospun microtubes. The encapsulated cells performed significant atrazine removal even under non-growth conditions bringing potential savings in process operation (Klein et al. 2012). Thus, the above reports