in which Az is the absorbance obtained at a defined pH and Aa and Ab are the absorbance of the acid and base form respectively. In this work, itwas chosen to calculate the pKa for each measured pH value and subsequently take the average
in which Az is the absorbance obtained at a defined pH and Aa andAb are the absorbance of the acid and base form respectively. In thiswork, itwas chosen to calculate the pKa for each measured pH valueand subsequently take the average