Brucella spp. isolates were recovered in tissues from 12 of the 13 dogs (Table 1), as well as from the placenta, fetal lung, and fetal liver. Of the 8 female dogs, isolates were most frequently obtained from the spleen (6/8) and uterus (6/8); less frequently in the sublumbar lymph nodes (4/7) and blood (4/8), and uncommonly in both mammary gland (1/7) and ovary (2/8). Of the 5 male dogs, only the prostate was consistently posi- tive (4/4), followed by the sublumbar lymph nodes (2/5) and epididymis (1/5). Bacteria were not recovered from the splenic tissue or blood of males. One male dog was culture-negative in all tissues submitted; however, the prostate from this dog was not tested.