by the combustion method (990.03) using
Leco equipment (Leco Corporation, St. Joseph, MI),
and crude fiber (method 962.09; AOAC, 2006) before
feed formulation, and the main results are reported
in Table 1. Expeller-extracted soybean meal samples
were sent to the laboratory of Mouriscade (Pontevedra,
Spain) to determine trypsin inhibitor levels, urease activity,
protein dispersibility index (PDI), and solubility
in KOH. Trypsin inhibitor activity, expressed in milligrams
of trypsin inhibited per gram of ESBM (mg/g),
was determined according to Kakade et al. (1974) as
modified by Hamerstrand et al. (1981). A sample (1 g)
of ESBM was ground using a laboratory grinder provided
with a 0.2-mm screen. After grinding, the ESBM
sample was defatted using ether.
by the combustion method (990.03) usingLeco equipment (Leco Corporation, St. Joseph, MI),and crude fiber (method 962.09; AOAC, 2006) beforefeed formulation, and the main results are reportedin Table 1. Expeller-extracted soybean meal sampleswere sent to the laboratory of Mouriscade (Pontevedra,Spain) to determine trypsin inhibitor levels, urease activity,protein dispersibility index (PDI), and solubilityin KOH. Trypsin inhibitor activity, expressed in milligramsof trypsin inhibited per gram of ESBM (mg/g),was determined according to Kakade et al. (1974) asmodified by Hamerstrand et al. (1981). A sample (1 g)of ESBM was ground using a laboratory grinder providedwith a 0.2-mm screen. After grinding, the ESBMsample was defatted using ether.
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