Patients were asked to wash and retain the respective
mouth rinse in the mouth for 40 Secs before expectorating
it. Patients were not allowed to consume any diet or drink
orally for following 90 mins. A salivary sample from each
patient was collected for pH, buffering capacity before
(pre-rinse) and after rinsing (post-rinse) at repeated
intervals of 10, 30, 60 and 90 mins however for microbial
analysis, saliva was collected only after 90 mins. The mouth
rinsing procedure and collection of salivary samples were
handled by one investigator and analysis of the salivary
parameters was carried out by different investigator who
was not aware of the experimental groups to blind the
study.