Furthermore, the increase in trypsin concentration (0–1.2 units/g shrimp shells) resulted in an increase in recovered proteins ( Klomklao et al., 2009). Trypsin was used to extract carotenoprotein from brown shrimp shell waste and showed the maximum recovery (55%) of carotenoid pigment in 4 h at (28±2 °C). Pepsin and papain yielded 50% recovery when the same hydrolysis time was used ( Chakrabarti, 2002). Armenta and Guerrero-Legarreta (2009) reported that fermented carotenoproteins from Pacific white shrimp waste were hydrolysed with a combination of protease and lipase. Protein recovery was used as an indicator for the cleavage of peptide bond, and the release of the carotenoprotein