For production of inhibitors, preinocula were grown in 10 mL of liquid LB (for strain MR1 and ALT3A) or ME (for RPT-82412) in 50 mL flasks for 48 h at 28 C shaken at 200 rpm. Production cultures were 50 mL in 250 mL flasks, inoculated with 1.0% (v/v) preinocula, and grown for 48 h at 28 C and 200 rpm.