Our previous studies on Vitex negundo leaf extract showed potential anti-implantation activity [10]. The leaf extract was further fractionated to evaluate this concept. Different fractions of Vitex negundo Linn leaf extract were screened for anti-implantation activity. V. negundo Linn is a large aromatic shrub from Verbenaceae family and is found throughout India especially in the warmer zones [11]. It has been claimed to possess many medicinal properties. Almost all of its parts are used in Indian systems of medicine to treat various diseases. Leaves and seeds of V. negundo were used traditionally for treatment of rheumatism and inflammatory disorder