Vinegar and mother of vinegar samples produced by the spontaneous fermentation method were obtained from three different local producers in Kayseri and Düzce, in Turkey. The samples, consisting of 2 grape vinegars (bg and eg) and their mothers (BG and EG), 2 apple vinegars (da and ha) and their mothers (DA and HA), were collected at the end of the acetic fermentation from wood barrels in which traditional surface fermentation is carried out. The acetic acid content of the vine-gar samples were reported by producers as 3.96, 4.08, 4.28 and 4.37℅ for the samples ha, da, bg and eg respectively. After the sampling process, the vinegar and mother of vinegar samples were analyzed for AAB using cultural and molecular methods.