The objective of the present study was to modify HPLC methods to used smaller sized column particles of 1.8e3 mm and to compare their performance with corresponding classicalHPLC methods using column particles of 5 mm. The methods were compared with respect to the time of elution, eluent consumption, separation of biogenic amines as well as recovery and repeatability for determination of biogenic amines in lean canned tuna and fatty frozen herring.