The process of cellulose formation in a nata-de-coco culture system has been investigated. The medium was prepared with coconut-water
by adding sugar and N-compounds and the culture was conducted in static conditions. The growth of gel thickness, wet weight and dry
weight was almost independent of the concentrations of N-compound and sugar at least when they were above 0.1 and 1%, respectively,
suggesting that the process was controlled by oxygen supply. Glucose was the only saccharide found in the coconut water stored for 3 days.
While the concentration of sugar dropped quickly to a certain level and decreased monotonically with time, a part of glucose turned to
something other than cellulose. No fructose was found after the initial stage, presumably being consumed by side-reactions. Computer
simulation showed that, after the induction time, the process of cellulose formation or the consumption of glucose is controlled by the
diffusion of atmospheric oxygen. q 1999 Elsevier Science Ltd. All rights reserved.
The process of cellulose formation in a nata-de-coco culture system has been investigated. The medium was prepared with coconut-waterby adding sugar and N-compounds and the culture was conducted in static conditions. The growth of gel thickness, wet weight and dryweight was almost independent of the concentrations of N-compound and sugar at least when they were above 0.1 and 1%, respectively,suggesting that the process was controlled by oxygen supply. Glucose was the only saccharide found in the coconut water stored for 3 days.While the concentration of sugar dropped quickly to a certain level and decreased monotonically with time, a part of glucose turned tosomething other than cellulose. No fructose was found after the initial stage, presumably being consumed by side-reactions. Computersimulation showed that, after the induction time, the process of cellulose formation or the consumption of glucose is controlled by thediffusion of atmospheric oxygen. q 1999 Elsevier Science Ltd. All rights reserved.
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