Fig. 1. Chromatograms of acrylamide obtained from different mobile phases and flow rates. (1) 10% (v/v) acetonitrile at 0.40 mL/min, (2) 15% (v/v) acetonitrile at 0.40 mL/min and (3) 10% (v/v) acetonitrile at 0.50 mL/min. The detection wavelength is 210 nm. Peaks with asterisks are acrylamide. The amount of the injected acrylamide standard is 10 ng. The chromatograms are offset for clarity.