The
amplification conditions used in this study were as follows: initial
denaturation for 11 min at 95 C followed by 35 cycles of 1 min at
94 C,1 min at 67 C, and 1 min at 72 C with a final 5 min extension
step at 72 C. The amplified samples were subjected to electrophoresis
on a 2.0% agarose gel.