Radical scavenging activity (RSA)
The ability of PJ to scavenge 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical was measured following the procedure described
by Karioti et al. with slight modifications . In Eppendorf tubes, PJ extract (15 l) was mixed with 735 l methanol and 0.1 mM solution of DPPH (750 l) dissolved in methanol. The mixture was incubated for 30 min in the dark at room temperature before measuring the absorbance at517 nm using a UV–visible spectrophotometer (Thermo ScientificTechnologies, Madison, Wisconsin). The RSA was determined by ascorbic acid standard curve (0–2000 M). The results were presented as micro molar ascorbic acid (AA) equivalent per millilitre of crude pomegranate juice (M AAE/ml PJ).