In order to define a well-established relationship among nucleotide sequence, deduced amino acid sequence and function, gene encoding beta-glucosidase from Pichia anomala MDD24 would be elucidated. Because the cost of cellobiose used for induction of this enzyme is very expensive, the extent heterologous expression of this enzyme in E. coli or some yeast strains to reduce production cost and obtain a sufficient amount is of practical significance in large-scale wine production.