High sensitivity testing agar (Hi-Media) was used for checking
antibacterial activity of crude protein extracts of different
plant seeds against S. aureus (ATCC 25923), E. coli (ATCC
25922), P. aeruginosa (ATCC 27853) and P. vulgaris (ATCC
6380). The microbial strains were cultured on the slants in
the sterilized Laminar Air Flow from the pure culture. These
cultured slants were incubated at 37 C for bacterial growth
for 2–3 days. High sensitivity testing agar was mixed at a concentration
of 23.4 g/1000 ml in distilled water and autoclaved
at 121 C for 15 min. A loop full from pure culture of a bacterial
strain was mixed in the 10 ml of Nutrient broth medium and incubated at 37 C overnight and the activated culture was
used for streaking onto the agar plates for antimicrobial
sensitivity.