These conditions were used to further screen 39 E. coli O157:H7, 24 E. coli non-O157, and 10
E. coli O157:non-H7 isolates (Table 1). The CLI assay produced true-positive recognition of 100% (39 of 39) of the
O157:H7 isolates, false-positive recognition of 100% (10 of 10) of the E. coli O157:non-H7 isolates, and false-positive recognition of 4.1% (1 of 24) of the E. coli non-O157 isolates tested