Aptamer is a kind of functional nucleic acid macromolecules which is made up of single-stranded DNA/RNA molecules that can be bind to a variety of non-nucleic acid target molecules with high specificity and affinity (Ellington and Szostak, 1990; Tuerk and Gold, 1990). Many selective aptamer-based PDGF-BB biosensors had been earlier reported. For example, (Tang et al., 2012) designed a simple colori- metric sandwich sensor for PDGF-BB detection with a low detection limit via dual enzyme processes to significantly amplify the signal, but the procedure was complicated. Wu et al. (2010) developed a new electrochemical aptameric sensing system for the detection of PDGF- BB with high selectivity, but the detection limit is not low enough. Moreover, these methods have only been operated effectively and