which often shows MT-loaded polar nuclear envelope invaginations
(Bajer and Mole-Bajer, 1969; Dixit and Cyr, 2002).
Such MT-dependent forces may participate in NEB, and these MTs may have a greater likelihood of encounter- ing kinetochores and building a spindle. NEB also causes direct accessibility of cytoplasmic proteins to chromatin. Histone H1-dependent nucleation may therefore participate in spindle formation in addition to the c–TuRC-dependent nucleation observed around chromatin and along pre- existing MTs. After NEB, an increasing number of proteins have been shown to participate in spindle formation (Figure 3).