Our objectives were to determine the antifungal effect of kefir fermented milk on
Aspergillus flavus AFUNL5 in vitro and to study if the addition of kefir fermented milk toarepas could produce shelf life improvement. We determined the antifungal effect on solid medium of kefir cell-free supernatants (CFS) obtained under different fermentation conditions Additionally, we compared the antifungal effect of kefir CFS with that obtained with unfermented milk artificially acidified with lactic plus acetic acids (lactic and acetic acids at the same concentration determined in kefir CFS) or with hydrochloric acid. Finally,kefir was added to the corn products either in the loaf recipe (kefir-baked arepas) or sprayed on to the baked-loaf surface (kefir-sprayed arepas). The loaves' resistance to natural and artificial fungal contamination and their organoleptic profiles were studied