In order to evaluate the interest of thosemembranes, we used them as reverse phase for protein extraction.We used mass spectrometry as the detection technique and bovine serum albumin (BSA) has been chosen as the biologicalmodel. The experiment consists in the filtration of a BSA aqueous solution onto different diamond membranes. Then, the retained proteins are eluted using a nonpolar solvent, namely acetonitrile. After elution, the collected eluent is characterized through LC tandem MS. The mean flow rate was deliberately set high in order to fit industrial process standards and was estimated at around 5 ml/min/cm2. The pore size of the pristine filter used as substrate for this experiment is of 0.7 μm. The holdup volume, calculated from the specification data of the glass fiber as provided by the supplier specifications, is 0.1895 cm3. One can note that the increase of the flow rate involves a decrease of N (cf. Eq. (1)), meaning that higher flow rates lead to lower breakthrough volume.