Control animals were injected with comparable amounts of10%ethanol in saline. Twenty intermolt
males (MMI 0) with an average weight of 13.5 1.4 g (SE) were used. Injections of dsRNA into the fifth walking leg sinus were performed on days 1, 3, 5, and 7, whereas ecdysone was injected daily until day 9. At the end of the experiment, all crayfish were anesthetized in ice-cold water and dissected.