Crude ovarian extracts and purified fractions from gel
filtration, ion-exchange chromatography and HPLC
were subjected to separation on 5–20% gradient native
polyacrylamide gel electrophoresis (PAGE) slab gels or
5–20% sodium dodecylsulfate–polyacrylamide gel elec-
trophoresis (SDS-PAGE) slab gels (Funakoshi Co. Ltd or
Daiichi Pure Chemicals Co. Ltd) according to Davis
16
and Laemmli.
17
To estimate the purity of Vt, native
PAGE slab gel was run using 0.05 M Tris-HCl buffer, pH
8.3, containing 0.384 M glycine. Sodium dodecylsulfate–