About 20 ml of the PCR product were analyzed by
electrophoresis in a 1% agarose gel (Sigma, USA)
with 0.5 TBE buffer (Sambrook et al., 1989) at
150 V for 45 min. DNA markers of Fermentas were
used as control. The bands were excised from the
gels and purified using GenElute Minus EtBr Spin
Columns (Sigma).