100 mL sample inserted into test tubes containing 3 ml of ethanol was 96% and divortex. Add 1 ml of DPPH in ethanol and in the vortex. Left for 30 minutes in a dark room at room temperature. Λ measured absorbance at 517 nm. Blanko are made by the same procedure, the sample was replaced with deionized distilled water. The inhibitory activity was calculated according to the equation.