This study was categorized as a completely randomized design and data were analyzed using PROC MIXED with additional programs used if main and/or simple effects required PROC SLICE with
the SAS statistical software version 9.2 (SAS Institute Inc., Cary,N.C.). Four replications of each experiment were included. Mean numbers of viable E. coli were statistically analyzed and treatment means were evaluated for statistically significant differences using the WallereDuncan test. Significant differences were defined at P < 0.05 for all experimental data.