Experiments were conducted in 250-ml Erlenmeyer
flasks, containing 15 g of substrate (dry weight basis)
and covered with eight layers of gauze. For all experiments,
initial conditions were: temperature, 30°C; pH,
6; inoculum size, 1107 spores:g dry matter (d.m.),
and initial water content, 70% (v:w) (maximum absorption
of the substrate). Experiments were performed to
study the effect of glucose addition (20, 35 and 46 g:100
g d.m.) on the production of volatile compounds, and
also the effect of addition of some possible precursors:
leucine (10 mmol), soya bean oil (10% v:w)) and a
saline solution, previously optimized [19]. All experiments
were conducted in three sets.