culture vials and transferred to half
strength MS medium with different
concentration (0.5-8.0 mg/l) of auxins like
IBA, IAA, 2,4-D, NAA for root induction.
The plantlets were taken out from culture
vessel without damaging the delicate root
system and rinsed with distilled water to
remove adhering agar and then transferred
to polycups containing vermicompost and
autoclaved soil (1:3). Polycups were
covered with inverted glass beakers to
maintain high humidity and kept in culture
chamber. They were gradually exposed
from artificial environmental conditions to
natural conditions for their acclimatization.