In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Here, it was shown that the expression of ANXA1 was observed mainly in the cytoplasm of the epithelial bile ducts, some inflammatory cells (large cell, macrophage-like cell), and tumor cells which increased with time as bile duct proliferation and CCA development progressed.