2.5. Comparative studies between different culture systems
Each NSB was inoculated with 60 explants. Total of 250 mL of liquid MS medium was sprayed at 1 h intervals. On the other hand the semi-solid and liquid culture systems contained 50 mL of liquid MS medium, and 12 explants in each vessel. The medium and culture conditions for the semi-solid and liquid culture systems were the same as those described above for the NSB system