The reaction mixture contained 500 mL of 2-deoxyribose (2.8 mM) in a potassium phosphate buffer
(50 mM, pH 7.4), 200 mL of premixed ferric chloride
(100 mM) and ethylene diamine tetraacetic acid (100 mM)
solution (1:1; v/v) and 100 mL of H2O2 (200 mM), either with
or without the extract (100 mL) in different concentrations
(15e500 mg).