P. temperata M1021 was identified and characterized from
soil entomopathogenic nematodes collected from the South
Korean locations reported in our previous study (KACC
accession number 91627P) (Jang et al., 2012). The bacterial
culture was routinely maintained in Luria-Bertani (LB)
broth (0.5% yeast extract, 1% NaCl, 1% tryptone) and incubated
at 28 ± 2°C for 48 h to prepare a pre-culture with an
initial optical density of 0.6 at 600 nm. The strain was cultured
on LB agar plant and in broth for both short- and
long-term storage and subsequent use in experiments.