RFLP or chromosomal DNA restriction analysis
Chromosomal DNA restriction analysis was the first ofthe chromosomal DNA-based typing schemes. Thebanding patterns that result after cutting and separatingthe DNA fragments by electrophoresis are referred toas DNA fingerprinting. Because of the high specificityof restriction enzymes and the stability of chromosomalDNA, a reproducible pattern of fragments is obtainedafter the complete digestion of the chromosomal DNAby a particular enzyme. These variations in thebanding patterns between strains are ascribed to basic