2.8. Antioxidant activity
The antioxidant activity was determined by the ABTS+ method,
as described by Rufino et al. (2010). The extract used for this analysis
was the same as that used for the determination of total
extractable polyphenols. The readings for the determination of
antioxidant activity before and after in vitro digestion were carried
out in a spectrophotometer (Shimadzu model UV-1800) to 734 nm,
and the quantification of the antioxidant activity before digestion
was performed on the extract prepared with the cashew apple
juice and the cashew apple fibre and the quantification after digestion
was performed in the dialysate. The quantification was performed
by using an external standard curve prepared with
Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic
acid) (Sigma Aldrich, Saint Louis, USA) as a reference. The results
were expressed as equivalent antioxidant Trolox (TEAC) in
lMg1. The bioaccessible percentage was calculated according to
Briones-Labarca et al. (2011) (Eq. (4)).
2.8. Antioxidant activity
The antioxidant activity was determined by the ABTS+ method,
as described by Rufino et al. (2010). The extract used for this analysis
was the same as that used for the determination of total
extractable polyphenols. The readings for the determination of
antioxidant activity before and after in vitro digestion were carried
out in a spectrophotometer (Shimadzu model UV-1800) to 734 nm,
and the quantification of the antioxidant activity before digestion
was performed on the extract prepared with the cashew apple
juice and the cashew apple fibre and the quantification after digestion
was performed in the dialysate. The quantification was performed
by using an external standard curve prepared with
Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic
acid) (Sigma Aldrich, Saint Louis, USA) as a reference. The results
were expressed as equivalent antioxidant Trolox (TEAC) in
lMg1. The bioaccessible percentage was calculated according to
Briones-Labarca et al. (2011) (Eq. (4)).
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