In conclusion, 10 Muscidae and 6 Sarcophagidae fly species collected in Korea were identifiable using COI sequence analysis. However, a few inconsistencies with previously reported sequences require further evaluation. To our knowledge, the present study provides the first report of the COI nucleotide sequences of H. occulta, M. angustifrons, M. pascuorum, O. leucostoma, S. haemorrhoidalis, P. harpax, and P. aureola.