Auditor:Please explain in details about the storage method after the purchase and the propagation method of the bacteria?
BOKEN:The purchased bacteria is a dried state. NB media is added to the dried bacteria and the bacteria is suspended in the NB media. The suspension is smeared on the plating medium by using a platinum loop. After incubation, take one colony and suspend in NB media and inoculate the suspension on the slant medium. After incubation, scrape bacteria, suspend in the maintenance medium, dispense in tube and store at -80 degree C.