Ten g portions of the crude extract which exhibited highest mortality of larvae was first fractionated on a silica gel (70-230 mesh) column chromatography and eluted with 20% step gradient of hexane in ethyl acetate and then with methanol.
Ten g portionsof the crude extract which exhibited highest mortality oflarvae was first fractionated on a silica gel (70-230 mesh)column chromatography and eluted with 20% step gradientof hexane in ethyl acetate and then with methanol.